Non-Ribosomal Peptide Biosynthesis and Engineering by Unknown

Author:Unknown
Language: eng
Format: epub
ISBN: 9781071632147
Publisher: Springer US

9.

Protein concentration column. For example, Amicon® Ultra-15 Centrifugal Filter Unit 100 kDa cut off column.

10.

A method to determine protein concentration. For example, A280 readings using a NanoPhotometer NP80 (Implen GmBH; Munich, Germany) (“Nanodrop”). Alternative methods such as the BCA assay may be used.

2.4 Kinetic Measurement of PPTase Activity

1.

Stock reagents.(a)

Sterile H2O.

(b)

1 M Tris-Cl pH 7.8.

(c)

1 M MgCl2.

(d)

100 mM ATP.

(e)

100 mM L-glutamine.

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